PDCD1 (Programmed Cell Death 1, PD-1, CD279) (PE)

Size

£767.00

SKU:: 145566
Application:: Flow Cytometry
Purity:: Purified by Protein A or G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse PDCD1, NS0-derived (UniProt # Q02242).
Clone:: 766104
Format:: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: Programmed Death-1 (PD-1) is type I transmembrane protein that belongs to the CD28/CTLA-4 family of immunoreceptors (1). In addition to PD-1, this family also includes CD28, CTLA-4, and ICOS (2-4). Signaling mediated by the CD28/CTLA-4 family regulates immune responses. PD-1 is most closely related to CTLA-4 and shares ~24% amino acid (aa) sequence identity. PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. Binding of PD-1 to PD-L1 or PD-L2 results in the inhibition of TCR-mediated proliferation and cytokine production as well as BCR-mediated signaling (1-4). Mice deficient in PD-1 have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. These observations collectively suggest that PD-1 likely has an inhibitory role in regulating immune responses. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice