Skip to content
  1. Shop all

145535

Mx1 (Interferon-regulated Resistance GTP-binding Protein MxA, MxA, IFI78, IFI-78K, OrthoMyxovirus-resistant Protein A, IFN-induced Protein p78/IFI-78K, Mx1)

Size

£889.00

SKU:
145535
Purity:
Purified by immunoaffinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Goat
Immunogen:
Recombinant protein corresponding to His412-Leu630 from human MxA/Mx1, expressed in E. coli.
Format:
Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:
MxA (orthoMyxovirus­resistant protein A; also IFN­induced protein p78/IFI­78K and Mx1) is a 75­78kD member of the Dynamin family, GTPase­binding superfamily of proteins. It is induced by type I and III interferons, and is known to reside in the cytoplasm and associate with the membranes of the ER. Typically, IFN­alpha is produced when influenza virus RNA (plus other viral dsDNA and RNA) is detected by internal Pattern recognition receptors (PRRs). IFN­alpha induces the synthesis of 300+ genes, one of which is MxA (Mx1 in mouse). In primates, MxA is cytoplasmic, and interacts with the influenza virus replication cycle at a point proximal to the transcription of genomic RNA into mRNA. By contrast, Mx1 in mouse acts in the nucleus to block primary viral gene transcription. Functionally, MxA is a GTPase, but it is only the binding of GTP to MxA that is associated with antiviral activity. Nucleotide binding to MxA is also associated with nonviral activity, being associated with the potentiation of TRPC Ca2+ channel activity. Human MxA is 662 amino acids (aa) in length. It contains one GTPase domain (aa69­340) and a GED (GTPase Effector Domain) over aa574­662. There are two ultilized phosphorylation sites at Tyr129 and Tyr451. MxA forms homo­dimers, ­tetramers and ­oligomers, with multimerization suggested to be important for activity. Although IFNs are typically considered to induce Mx gene expression, HSV­1 itself also activates gene transcription. In this case, however, a truncated 54­57kD transcript is generated that contains an 84 aa substitution for aa425­662. Over aa412­630, human MxA shares 49% aa sequence identity with mouse Mx1. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Human peripheral blood mononuclear cells (PBMC) treated with Recombinant Human IFN‑α Universal Type I and LPS Immunohistochemistry: 5­-15ug/ml Immersion fixed paraffin-embedded sections of human thymus Immunocytochemsitry: 1-15ug/ml Immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with IFN-alpha Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice