Ly6K (Lymphocyte Antigen 6 Locus K, CO16, CT97)

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£863.00

SKU:: 145516
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Met1-Gly138 from human Ly6K, expressed in CHO cells.
Clone:: 750747
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% Trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Ly6K (Lymphocyte antigen 6 locus K; also CO16) is a 26-27kD member of the Ly-6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor-patient blood. Mature human Ly6K is a 121 amino acid (aa) GPI-linked (presumed) glycoprotein. It is synthesized as a 165aa preproprecursor that contains a 17aa signal sequence, a 121aa mature region (aa18-138), and a 27aa C-terminal propeptide. There are two potential splice variants, one that shows a 48 aa substitution, and another that shows a 33aa substitution for aa73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa18-138, human Ly6K shares 39% aa identity with mouse Ly6K. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells using HeLa human cervical epithelial carcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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