145508
LPHN3 (Latrophilin 3, CIRL3, LEC3, Lectomedin-3)
Size
£787.00
- SKU:
- 145508
- Purity:
- Purified by Protein A or G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1-813 from human LPHN3, CHO-derived (UniProt # Q9HAR2).
- Clone:
- 801518
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- LPHN3 (Latrophilin 3; also CIRL3, CL3 and LEC3) is a (presumably) 220kD member of the LNTM7 subfamily, GPCR 2 family of molecules. It appears to have a restricted expression pattern, being limited to brain and adrenal gland. Although it is related to the black widow toxin receptor CIRL1, it does not serve as a toxin receptor. Mature human Latrophilin 3 is a 7TM glycoprotein that is 1428 amino acids (aa) in length. Posttranslational processing cleaves the molecule into a 120kD ECD (aa20841) and a noncovalentlyassociated 85kD 7TM Cterminus (aa8421447). The ECD is modular and contains a SUELlike lectin domain (aa 35124), an Olf region (aa134393) and GPS domain (aa 802853). There are multiple splice events which, in the ECD, include a two aa substitution for aa127131, a 13aa insertion after aa623, and a 39aa substitution for aa6681447. Over aa20832, human Latrophilin 3 shares 98% aa identity with mouse Latrophilin 3. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice