145498
IRF8 (Interferon Regulatory Factor 8, ICSBP1)
Size
£825.00
- SKU:
- 145498
- Purity:
- Purified by Protein A or G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa255-426 from human IRF8 expressed in E. coli (UniProt # Q02556).
- Clone:
- 809926
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- IRF8 (interferon regulatory factor 8; also ICSBP) is a 50kD member of the IRF family of proteins. It is expressed in myeloid, dendritic and B cells and interacts with PU.1 to regulate the expression of TLR4, IL18, IL12p35, and PTPN13, a Fasassociated phosphatase that blocks Fasinduced apoptosis. Human IRF8 is 426 amino acids (aa) in length. It contains an Nterminal DNAbinding basic region (aa9110) and at least one regulatory phosphorylation site at Tyr95. Over aa255426, human IRF8 shares 90% and 88% aa sequence identity with canine and mouse IRF8, respectively. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunohistochemistry: 825ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice