145493
TCCR (T Cell Cytokine Receptor, IL-27 R alpha, IL27RA, WSX-1) (APC)
Size
£863.00
- SKU:
- 145493
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gly34-Lys516 of human TCCR, expressed in NS0 cells.
- Clone:
- 191106
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- IL-27 Ralpha, also known as TCCR (T cell cytokine receptor) and WSX-1, is a 96kD member of the type I group 2 cytokine receptor family. The extracellular region of human TCCR shares 63% amino acid identity with the extracellular domain of mouse IL-27 Ralpha.2, 3 TCCR is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and naïve T cells. As with many other class I cytokine receptor chains, IL-27 Ralpha heterodimerizes with gp130, the common receptor subunit of IL-6 family cytokines, to form a functional IL-27 receptor. IL-27 only binds to IL-27 Ralpha/WSX-1/TCCR and not the gp130 subunit.7 Studies using WSX-1 mice reveal that WSX-1 has the ability to suppress T cell hyperactivity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity. In particular, WSX-1 is believed to induce IL-12 R on naïve T cells, predisposing them to a Th1 phenotype. This is offset by its ability to limit IL-12 (and TNF) production by macrophages, and its suppression of IFN-gamma production. Applications: Suitable for use in Flow Cytometry. Other applications not tested Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells of human peripheral blood monocytes Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap..
- Shipping Conditions:
- Blue Ice