145490
IFI30 (GILT, Gamma-Interferon-inducible Lysosomal Thiol Reductase, IP30, Legumaturain)
Size
£812.00
- SKU:
- 145490
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HEK293 human embryonic kidney cell line transfected with human IFI30, Ser27-Lys250.
- Clone:
- 844623
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- IFI30 (Gammainterferoninducible protein IP30; also gammainterferon [IFNgamma] inducible lysosomal thiol reductase/GILT and Legumaturain) is a 25-30kD member of the GILT family of proteins. It is constitutively expressed in B cells and dendritic cells, and induced by IFN-gamma in non-APCs. IFI30 is both intracellular and secreted as an inactive glycosylated proenzyme. The glycosylation pattern contains a terminal phosphorylated mannose, which is recognized by cell surface mannose-6 phosphate receptors and internalized into lysosomes. In lysosomes, IFI30 is processed into an active, mature form, and via a thiol reductase domain, breaks disulfide bonds in molecules destined for lysosomal degradation. This is a critical first step in the processing and subsequent presentation of peptides that will initiate an antigenic response. The human IFI30 proenzyme is 224 amino acids (aa) in length. In this form, it is 3335kD in size. Following proteolytic processing at both the N- and C-terminus, a 175aa, 2530kD active mature form is generated (aa58232). The mature region possesses a thiol reductase domain (aa62151) plus one utilized Thr phosphorylation site. Both the pro and mature forms exhibit enzymatic activity. IFI30 is known to exist as a 50-60kD disulfidelinked homodimer. There are four potential isoform variants. One contains a 26aa substitution for aa213250, a second shows a deletion of aa131-161, a third shows a deletion of aa106-123, while a fourth shows a deletion of aa64-212. Over aa27-250, human IFI30 shares 62% aa sequence identity with mouse IFI30. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using Raji human Burkitt’s lymphoma cell line Immunocytochemistry: 5-25ug/ml using immersion fixed THP-1 human acute monocytic leukemia cell line and MCF-7 human breast cancer cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice