145482
GPHN (Gephyrin, Molybdopterin Adenyltransferase, GEPH, GPH, GPHRYN)
Size
£800.00
- SKU:
- 145482
- Application:
- WB
- Purity:
- Purified by Protein A or G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa2-188 from human Gephyrin/GPHN, expressed in E. coli (UniProt # Q9NQX3).
- Clone:
- 807423
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Gephyrin (GPHN, from molybdopterin adenyltransferase) is a 9094kD member of both the MoaB and MoeA families of proteins. It has limited expression, being found in hepatocytes and neurons. In neurons, gephyrin is a postsynaptic density cytoplasmic protein that interacts with the subunits of glycine and GABAA receptors. This interaction is mediated by the ability of gephyrin to form hexameric lattices that act as scaffolds that link the subunits of the two inhibitory receptors to the cell's underlying tubulin network. In addition, the gephyrin lattice may also interact with NLG1 and 2, suggesting a role for gephyrin in the regulation of glutamatergic synapses. Gephyrin is also known to participate in the synthesis of molybdenum cofactor. This cofactor binds molybdenum, making it available to sulfite and aldehyde reductase. Human gephyrin is 736 amino acids (aa) in length. It contains a 181aa Nterminal Gdomain that possesses MPT Motransferase activity (aa14166) and a homotrimer interface motif, followed by a 420aa Cterminal region (aa319738) that contains an MPT adenyltransferase domain (aa326736) with an embedded homodimerization motif. There are at least five utilized Ser/Thr phosphorylation sites. Multiple splice variants exist, and represent peptide insertions at multiple sites. There is a 13aa insertion after Glu98, a 33aa insertion after Ser243, and 15, 19, 21 and 24aa insertions after Lys288. Over aa2188, human and mouse Gephyrin are identical in amino acid sequence. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice