GCP3 (NAALADase-2, GCPIII, Glutamate Carboxypeptidase III, NAALAD2, N-acetylated-alpha-linked Acidic Dipeptidase 2)

Size

£825.00

SKU:: 145474
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Lys32-Leu740 from human GCP3, expressed in CHO cells
Clone:: 756030
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul of sterile PBS.
Extra Details:: Nacetylatedalphalinked acidic dipeptidase 2 (NAALADase2), also known as glutamate carboxypeptidase 3 (GCP3), is a 90-100kD type 2 transmembrane protein in the M28 peptidase family. It hydrolyzes betacitrylglutamate (BCG) and Nacetylaspartylglutamate (NAAG) which are abundant dipeptides found in brain and testis. Mature human NAALADase2 consists of a 7 amino acid (aa) cytoplasmic domain, a 24aa transmembrane segment, and a 709aa extracellular region (aa32-740) that contains the peptidase domain. Over aa32-740, human NAALADase2 shares 89% aa sequence identity with mouse NAALADase2. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human testis Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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