145449
DSC3 (Desmocollin-3, CDHF3, DSC-3, DSC4, HT-CP)
Size
£800.00
- SKU:
- 145449
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-3 Arg136-Leu695.
- Clone:
- 765129
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute in 200ul sterile PBS.
- Extra Details:
- DSC3 (Desmocollin [Greek for "gluethatbinds"]3) is a 95110kD member of the Ca++dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including keratinized and nonkeratinized epithelia of the urinary bladder, vagina, oral cavity and skin. DSC3 serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, in the extracellular space, DSC3 forms both homotypic and heterotypic interactions with DSG1 intrans, and binds to the cytoskeleton intracellularly via plakophilin3. Mature mouse DSC3 is a 760 amino acid (aa) type I transmembrane glycoprotein. The mature molecule contains a 560aa extracellular region with five cadherin domains (aa136691), and a 179aa cytoplasmic domain that possesses three utilized Ser phosphorylation sites. There is one splice variant that shows an eight aa substitution for aa831895. Over aa136695, mouse DSC3 shares 79% and 93% aa identity with human and rat DSC3, respectively. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice