145439
COTL1 (CLP, Coactosin-like Protein)
Size
£856.00
- SKU:
- 145439
- Purity:
- Purified by immunoaffinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Ala2-Glu142 from human COTL1, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute in 500ul sterile PBS.
- Extra Details:
- COTL1 (Coactosin-like Protein) is both a cytoplasmic and plasmaappearing 15-16kD member of the coactosin subfamily, ADF/Actin Depolymerizing Factor family of actin-binding proteins. It is widely expressed, and found in cell types such as neutrophils, and tissues such as placenta, lung and kidney. Functionally, COTL1 interacts noncovalently with both F-actin and 5lipoxygenase/5LO. These interactions appear to be mutually exclusive. A COTL1:Factin interaction leads to actin binding without actin polymerization, while a 5LO:COTL1 interaction has two potential outcomes; first, 5LO sequesters COTL1, leading to a failure of actin binding, and second, COTL1 can serve as a scaffold for 5LO activity, facilitating the production of either 5HPETE or LTA4. Human COTL1 is 142 amino acids (aa) in length. It is principally composed of one ADF-H domain (aa2-130) that possesses a utilized phosphorylation site at Ser115, and two acetylation sites at Lys102 and Lys126. COTL1 may form noncovalent homodimers and oligomers, but not when complexed to Factin. There is one potential isoform variant that shows a 106aa substitution for aa1-53. Full-length human and mouse COTL1 share 95% aa sequence identity. Applications: Suitable for use in Direct ELISA, Western Blot, and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1ug/ml using JAR human choriocarcinoma cell line, NCL-H345 human small cell lung carcinoma cell line, human placenta tissue and rat placenta tissue Immunocytochemistry: 5-15ug/ml using immersion fixed NCI-H128 human small cell lung carcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice