Cadherin-11 (CDH11, CDHOB, OB-Cadherin) (PE)

Size

£741.00

SKU:: 145401
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Phe23-Thr617 from human Cadherin-11, expressed in NS0 cells
Clone:: 667039
Format:: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: The Cadherin superfamily comprises a large number of membrane glycoproteins with one or more Cadherin repeats, which are involved in Ca2+ dependent cell-cell adhesion. The family can be subdivided into several major subgroups, including the type I and type II classical Cadherins, desmosomal Cadherins, protocadherins, seven transmembrane (Flamingo) Cadherins, FAT-family Cadherins, T-Cadherin, and other unclassified Cadherins. Cadherin-11, also known as OB-Cadherin, is a type II classical Cadherin. Classical Cadherins are type I transmembrane proteins with an N-terminal extracellular domain containing five tandem Cadherin repeats and a C-terminal cytoplasmic domain with a characteristic sequence for binding to catenins. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells using PC-3 human prostate cancer cell line stained in buffer containing Ca2+ and Mg2+ Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice