145397
BAD, phosphorylated (S99) (Bcl-2 Antagonist of Cell Death, BBC6, BCL2L8)
Size
£761.00
- SKU:
- 145397
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Phosphopeptide containing the human Bad S99 site.
- Clone:
- 679431
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Bcl-2 antagonist of cell death (Bad) is an 18kD cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of Bad on Ser75, Ser99, and Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Phosphorylation of Ser75 and Ser99 is also required for the ability of Bad to induce cell cycle arrrest in G1. Human Bad shares 75 % aa sequence identity with mouse and rat Bad. Applications: Suitable for use in ELISA and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 8-25ug/ml in immersion fixed HEK293 human embryonic kidney cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice