BAD (Bcl-2 Antagonist of Cell Death, BBC6, BCL2L8)

Size

£748.00

SKU:: 145396
Application:: WB
Purity:: Purified by Protein A or G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse Bad, E. coli- derived
Clone:: 64103
Format:: Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Extra Details:: The Bcl-2 family of proteins consists of both inhibitors and promoters of apoptosis. The relative ratios of pro- and anti-apoptotic Bcl-2 family proteins determine the sensitivity or resistance of cells to a variety of apoptotic signals. The pro-apoptoptic Bad (Bcl-2-antagonist of cell death) heterodimerizes strongly with Bcl-x, less strongly with Bcl-2, and not at all with Bax, Bcl-xS, Mcl-1, A1, or itself. When Bad dimerizes with Bcl-xL, Bax is displaced and its apoptosis-inducing ability is restored. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.