145388
AMBP (Alpha 1-Microglobulin, A1M, AMBP, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI)
Size
£787.00
- SKU:
- 145388
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Asp20-Ala202 from rat alpha 1-Microglobulin, expressed in E. coli.
- Clone:
- 771803
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Rat alpha 1Microglobulin (alpha1m/A1M; also protein HC) is a secreted 2526kD glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. Alpha 1Microglobulin appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. Alpha 1Microglobulin circulates either as a monomer, or bound to IgA, albumin or prothrombin. Rat alpha 1Microglobulin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP1, a 120140kD adrenomedullinbinding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an Nterminal 183aa alpha 1Microglobulin protein (aa20202), and a Cterminal serine protease inhibitor termed bikunin (aa205349). Alpha 1Microglobulin possesses one lipocalin domain (aa41186). Although cleavage of AMBP in the Golgi apparatus typically generates a 25kD alpha 1Microglobulin and 28kD bikunin molecule, the 5565kD AMBP precursor can also be released intact. In human, alpha 1Microglobulin will undergo extracellular processing, generating an isoform that is missing the Cterminal four amino acids. There are four potential isoform variants. One utilizes an alternative start site at Met181, a second contains an Asn substitution for aa 112228, a third possesses an 11aa substitution for aa1141, and a fourth shows a 17aa substitution for aa201349. Over aa20202, rat alpha 1Microglobulin shares 76% and 86% aa sequence identity with human and mouse alpha 1Microglobulin, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Simple Western: 20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice