ACVR1C (ALK-7, Activin Receptor-like Kinase 7, Activin RIC, ACVRLK7)

Size

£812.00

SKU:: 145379
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Leu26-Glu113 from human ALK‑7, expressed in CHO cells.
Clone:: 810506
Format:: Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Activin receptor-like kinase 7 (ALK-7), also known as Activin R1C (gene name ACVR1C), is a glycosylated 58kD type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. It associates with type II receptors to form a signaling complex that responds to the ligands Activin AB, and Activin B, GDF3, and Nodal. ALK-7 plays a role in regulating energy balance by inhibiting insulin secretion and inducing pancreatic beta cell apoptosis. It is expressed in adipose tissue but downregulated in obesity. ALK-7 is also expressed in pituitary gonadotropic cells and in pre-eclamptic placenta. It induces the apoptosis of trophoblasts as well as ovarian granulosa and epithelial cells. Within the extracellular domain, human ALK-7 shares 95% and 91% amino acid (aa) sequence identity with mouse and rat ALK-7, respectively. Alternate splicing of human ALK-7 generates additional isoforms with either a 50aa N-terminal truncation or with deletions of 79aa or 157aa that encompass the transmembrane segment. Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells using PC-3 human prostate cancer cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice