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145378

Acid Phosphatase 1 (LMW-PTP, HAAP, Low Molecular Weight Protein Tyrosine Phosphatase, Red cell Acid phosphatase 1, ACP1, HCPTP, HAAP, Adipocyte Acid Phosphatase)

Size

£843.00

SKU:
145378
Application:
WB
Purity:
Purified by immunoaffinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Sheep
Immunogen:
Recombinant protein corresponding to Ala2-His158 from human LMW‑PTP/ACP1, expressed in E. coli.
Format:
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:
LMW­PTP (Low Molecular Weight Protein Tyrosine Phosphatase; also Red cell acid phosphatase 1/ACP1, HCPTP, and HAAP/Adipocyte acid phosphatase) is a 17­18kD member of the LMWPTP family, PTP superfamily of enzymes. It is widely expressed, being found in cells diverse as B and T cells, endothelial cells and vascular smooth muscle cells. LMW­PTP dephosphorylates multiple substrates, often in a cell­dependent manner. Among the substrates for LMW­PTP include PDGR beta, EphA2 and beta­catenin. PMW­PTP is found intracellularly, generally associated with two cytoplasmic pools, one in the cytosol and the other accompaning the cytoskeleton. Human LMW­PTP is 158 amino acids (aa) in length. The catalytic region spans aa9­154, and there are two acetylation sites at Ala2 and Lys156. Phosphorylation on Tyr132 and Tyr133 occurs on cytoskeleton­associated LMW­PTP, and has the effect of increasing its phosphatase activity. There are three alternative splice variants reported for LMW­PTP. One shows a 34aa substitution for aa41­74, a second shows a deletion of aa41­74, while a third contains a 35aa substitution for aa78­158. The last two isoforms may function a negative regulators of LMW­PTP activity. Full­length LMW­PTP shares 86% aa sequence identity with mouse LMW­PTP. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice