144992
NRF1 (Alpha palindromic-binding protein)
Size
£620.00
- SKU:
- 144992
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C/-20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa272-288, QHGREDLLYAFEDQQTQ of human NRF1. Identical to the related rat and mouse sequences.
- Format:
- Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstiute with 200ul sterile ddH2O.
- Extra Details:
- NRF1 (Nuclear Respiratory Factor 1), also known as Alpha-Pal. Gopalakrishnan and Scarpulla (1995) analyzed DNA from a panel of human/hamster cell hybrids using human-specific NRF1 PCR primers and localized the NRF1 gene to human chromosome 7. The assignment was further refined to 7q31 by cohybridization of NRF1- and chromosome 7-specific probes to human metaphase chromosomes. Efiok et al. (1994) identified genes containing alpha-Pal-binding sequences and found that these could be classified either as cellular proliferation genes, or as genes regulating the growth-responsive metabolic pathways of energy transduction, translation, and replication. Virbasius and Scarpulla (1994) noted that the nuclear-encoded mitochondrial transcription factor TFAM contains potential binding sites for NRF1, NRF2 (GABPA) and SP1 within the promoter region. With use of binding and electrophoretic mobility shift assays, DNase footprinting, and mutation analysis of recombinant proteins, they demonstrated specific and functional binding of NRF1 and NRF2 to the TFAM promoter region. Applications: Suitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.5ug/ml Immunohistochemistry(Paraffin): 0.5-1ug/ml. Boil paraffin sections in 10mM citrate buffer, pH 6.0, for 20 min is required. Immunocytochemistry: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice