144946
NADPH--cytochrome P450 reductase (POR)
Size
£620.00
- SKU:
- 144946
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C/-20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A synthetic peptide corresponding to a sequence at the C-terminal of human POR, different from the related rat and mouse sequences by one amino acid.
- Format:
- Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitutie with 200ul sterile ddH2O.
- Extra Details:
- POR (Cytochrome P450 Oxidoreductase), also known as Cytochrome P450 Reductase, is a membrane-bound enzyme required for electron transfer to cytochrome P450 in the endoplasmic reticulumof the eukaryotic cell from a FAD- and FMN-containing enzyme NADPH: cytochrome P450 reductase. By Southern blot analysis of DNA isolated from a panel of 8 independent human-rodent somatic cell hybrids, Shephard et al. (1989) determined that cytochrome P450 reductase is encoded by a single gene located on 7pter-q22. By in situ hybridization to metaphase chromosomes, they refined the localization to 7q11.2. Cytochrome P450 oxidoreductase is a flavoprotein that donates electrons to all microsomal P450 enzymes, including the steroidogenic enzymes P450c17 (CYP17A1), P450c21 (CYP21A2), and CYP51A1 (Miller, 1986). UniProt Number: P16435 Gene ID: POR Applications: Suitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice