MIP3A (Macrophage Inflammatory Protein 3 alpha, MIP-3-alpha, MIP-3a, Beta Chemokine Exodus-1, CC Chemokine LARC, C-C Motif Chemokine 20, Chemokine (C-C Motif) Ligand 20, CCL20, CKb4, Liver and Activation-regulated Chemokine, LARC, Small-inducible Cytokine A20, SCYA20, ST38)

Size

£466.00

SKU:: 143657
Purity:: Purified by Protein A affinity chromatography from cell culture.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human MIP3A expressed in E. coli.
Clone:: 14G19
Format:: Supplied as a lyophilized powder from PBS. Reconstitute with 500ul of sterile ddH2O.
Extra Details:: Chemokines are a family of related proteins that regulate leukocyte migration (including into tissues) and activation in the face of inflammatory stimulus. CCL20/LARC/EXODUS/MIP-3 has been shown to be highly expressed in liver, and preferentially in lymphocytes and monocytes where its expression is upregulated by mediators of inflammation. Applications: Suitable for use in ELISA and Western Blot. Other applications have not tested. Recommended Dilution: ELISA (Sandwich): 2-4ug/ml using 100ul/well antibody solution in conjunction with biotinylated anti-human MIP3A (1-2ug/ml, M1202-63A) as a detection antibody, allows the detection of at least 1000pg/ml of recombinant human MIP3A. Western Blot: 0.2-0.4ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human MIP3A is 0.25-0.5ng/lane under non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice