CXCL10 (IP-10, C-X-C Motif Chemokine 10, 10kD Interferon gamma-induced Protein, Gamma-IP10, Small-inducible Cytokine B10, INP10, SCYB10)

Size

£466.00

SKU:: 143638
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human IP-10 expressed in E. coli.
Clone:: 14G4
Format:: Supplied as a lyophilized powder from PBS. Reconstitute with 500ul sterile ddH2O.
Extra Details:: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. Applications: Suitable for use in Sandwich ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Sandwich ELISA: 1-2ug/ml (assuming 100ul/well) will detect at least 1000pg/ml of recombinant Human IP-10 when used with affinity purified anti-human IP-10 (Biotin) as the detection antibody at a concentration of ~0.25ug/ml. Western Blot: 0.25-0.5ug/ml (non-reducing) or 1-2ug/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant human IP-10 is 0.24ng/lane under non-reducing conditions and 3.91 under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.