143620
IL22 (Interleukin-22, IL-22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible Factor, IL-TIF, ILTIF, ZCYTO18, UNQ3099/PRO10096, MGC79382, MGC79384)
Size
£626.00
- SKU:
- 143620
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human IL22 expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
- Extra Details:
- Cytokine that contributes to the inflammatory response in vivo. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications have not been tested. Recommended Dilutions: ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution) In conjunction with biotinylated anti-human IL22 (I8443-18H) as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human IL22. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-22 is 1.5-3ng/lane, under either reducing or non-reducing conditions. Neutralization: 0.4ug/ml yields one-half maximal inhibition of the biological activity of human IL-22 (3ng/ml). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice