143616
Il2 (Interleukin-2, IL-2, T-cell Growth Factor, TCGF, Aldesleukin)
Size
£453.00
- SKU:
- 143616
- Purity:
- Purified by Protein G affinity chromatography from cell culture.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to rat IL-2, expressed in E. coli.
- Clone:
- 14G13
- Format:
- Supplied as a lyophilized powder. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 4-8ug/ml of this antibody will detect at least 1000pg/ml of recombinant rat IL-2 when used with biotinylated antigen affinity purified anti-rat IL-2 as the detection antibody at a concentration of at least 2-4ug/ml. Western Blot: To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.5ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2-4ng/lane, under reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice