Il2 (Interleukin-2, IL-2, T-cell Growth Factor, TCGF, Aldesleukin)

Size

£435.00

SKU:: 143615
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse IL-2, expressed in E. coli.
Clone:: 14G12
Format:: Supplied as a lyophilized powder. Reconstitute with sterile PBS to 0.1-1mg/ml
Extra Details:: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 4-8ug/ml of this antibody will detect at least 32pg/ml of recombinant murine IL-2 when used with biotinylated antigen affinity purified anti-murine IL-2 as the detection antibody at a concentration of at least 1-2ug/ml. Western Blot: To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2-4ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1-2ng/lane, under reducing conditions and 0.2-0.4ng/lane, under non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice