143589
IFNG (Interferon gamma, IFN-gamma, Immune Interferon)
Size
£416.00
- SKU:
- 143589
- Purity:
- Purified by Protein A affinity chromatography from cell culture.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human IFN-g, expressed in E. coli.
- Clone:
- 14G7
- Format:
- Supplied as a lyophilized powder. Reconstitute with sterile ddH2O.
- Extra Details:
- Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 4-8ug/ml of this antibody will detect at least 500pg/ml of recombinant human IFN-gamma when used with biotinylated antigen affinity purified anti-human IFN-gamma as the detection antibody at a concentration of approximately 2-4ug/ml. Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1-3ng/lane, under non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice