Galectin-1 (GAL1, LGALS1, GAL-1, Lectin Galactoside-binding Soluble 1, Beta-galactoside-binding Lectin L-14-I, Lactose-binding Lectin 1, S-Lac Lectin 1, Galaptin, 14kD Laminin Binding Protein, 14kD Lectin, HLBP14, HPL, HBL, Putative MAPK-activating Protein PM12, GBP) (Biotin)

Size

£479.00

SKU:: 143575
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human Galectin-1, expressed in E. coli.
Format:: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to 0.1-1mg/ml. Labeled with Biotin.
Extra Details:: Human galectin-1 (Gal-1), a member of the galectin family of beta-galactoside-binding proteins, which exhibits both intracellular and extracellular functions, and is differentially expressed by a wide range of normal and neoplastic tissues. Gal-1 has been implicated in several major cellular functions including proliferation, motility, hematopoietic cell differentiation, apoptosis promotion and immunoregulatory effects. The expression of Gal-1 by neuronal and glial cells correlates with neuroregeneration following injury, and studies have shown the importance of Gal-1 to primary sensory neurons and synaptic connections in the spinal cord, during embryonic development. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (sandwich): 0.25-1ug/ml (using 100ul/well antibody solution). In conjunction with 143574 as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGalectin-1. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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