143563
Endostatin (Collagen alpha-1(XVIII) Chain, COL18A1)
Size
£576.00
- SKU:
- 143563
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Highly purified human recombinant Endostatin, expressed in E. coli
- Format:
- Supplied as a liquid in PBS, pH 7.2.
- Extra Details:
- Endostatin is a naturally occurring 20kD polypeptide derived from the C-terminal portion of type XVIII collagen. It functions as an anti-angiogenic cytokine that is expressed in various organs with the highest levels in liver, lung and kidney. Endostatin inhibits angiogenesis by blocking the pro-angiogenic activities of VEGF and FGF-basic. Recombinant human Endostatin is a 20.2kD protein consisting 184aa residues. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution. Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human Endostatin is 1.5-3ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: 0.125-0.25ug/ml with an overnight incubation at 4°C stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice