NT-3, Recombinant, Human (Neurotrophin-3, Neurotrophic Gactor, Nerve Growth Factor-2, NGF-2, HGNF)

Size

£332.00

SKU:: 143454
Molecular Weight:: 27.2
Purity:: ≥98% by SDS-PAGE gel and HPLC analyses. Endotoxin: ≤0.1ng/ug (<1EU/ug)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM PBS, pH 7.2. Reconstitute with sterile PBS, 0.1% BSA to 0.1mg/ml.
Extra Details:: NT-3 is a neurotrophic factor structurally related to beta-NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical. Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kD polypeptide monomers (240 total amino acid residues). Human and mouse NT-3 sequences are identical. Biological Activity: The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50ng/ml. AA Sequence: MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT Biological Activity: Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice