143416
INSL5/INSL7 Hybrid, Recombinant, Human
Size
£301.00
- SKU:
- 143416
- Molecular Weight:
- 5.2
- Purity:
- ≥98% (SDS-PAGE gel and HPLC). Endotoxin: ≤0.1ng/ug of protein (<1EU/ug).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to 0.1-1mg/ml
- Extra Details:
- The Insulin/Relaxin structural family of proteins consists of at least seven members, each containing an insulin-like heterodimeric structure of two polypeptide chains held together by two inter-chain disulfide bonds. The INSL5/INSL7 hybrid contains the β-chain of INSL7 (Relaxin-3), linked to the α-chain of INSL5. The INSL5/INSL7 hybrid protein can specifically activate the G protein-coupled receptors, GPCR135 and GPCR142, but unlike Relaxin-3, does not induce significant activation of the LGR7 receptor. Recombinant protein corresponding human INSL5/INSL7 hybrid, consisting of a 27aa β-chain linked to a 21aa α-chain by two disulfide bonds, expressed in E. coli. Amino Acid Sequence: A-Chain: QDLQTLCCTD GCSMTDLSAL C B-Chain: RAAPYGVRLC GREFIRAVIF TCGGSRW Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice