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143412

IL-7, Recombinant, Rat (Lymphopoietin 1, LP-1, pre-B cell factor)

Size

£320.00

SKU:
143412
Purity:
≥98% (SDS-PAGE, HPLC). Endotoxin: ≤0.1ng/ug
Storage Conditions:
-20°C
Supplier:
United States Biological
Format:
Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile ddH2O to 0.1mg/ml. For extended storage, the addition of 0.1% BSA is recommended.
Extra Details:
IL-7 is a hematopoietic growth factor which affects primarily early B and T cells.Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2.Human and murine IL-7 is cross-species reactive. Recombinant rat IL-7 is a 15.0kD protein containing 130 amino acid residues. Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is ~2.0ng/ml, corresponding to a specific activity of ~5x10e6 units/mg. AA Sequence: MDCHIKDKDG KAFGSVLMIS INQLDKMTGT DSDCPNNEPN FFKKHLCDDT KEAAFLNRAA RKLRQFLKMN ISEEFNDHLL RVSDGTQTLV NCTSKEEKTI KEQKKNDPCF LKRLLREIKT CWNKILKGSI Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. For extended storage, the addition of 0.1% BSA is recommended. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice