IL-3B, Recombinant, Rat (MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor)

Size

£364.00

SKU:: 143408
Molecular Weight:: 16.3
Purity:: ≥98% by SDS-PAGE gel and HPLC analyses. Endotoxin: ≤0.1ng/ug (<1EU/ug).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 5mM sodium citrate, pH 4.0. Reconstitute with sterile ddH2O to 0.1-1mg/ml
Extra Details:: IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages.Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity.Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles.Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant rat IL-3beta is a 16.3kD globular protein containing 144 amino acid residues. Molecular Weight: ~16.3kD Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of thymidine uptake by murine MC-9 cells is ~10ng/ml, corresponding to a specific activity of ~1.0x10e5 units/mg. Amino Acid Sequence: MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice