GDF-5, Recombinant, Human (BMP-14, Cartilage-Derived Morphogenetic Protein-1 (CDMP-1))

Size

£338.00

SKU:: 143344
Molecular Weight:: 27
Purity:: ≥98% by SDS-PAGE gel and HPLC analyses. Endotoxin: ≤0.1ng/ug (≤1EU/ug).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 10mM sodium citrate, pH 3.5. Reconstitute with ddH2O to a concentration of 0.1-1.0mg/ml. Do not vortex.
Extra Details:: GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Grebe Syndrome, which is characterized by short stature, extra digits, short and deformed extremities, and in Hunter-Thompson type dwarfism. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino-acid signal peptide and a 354 amino-acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease. Recombinant human GDF-5 is a 27.0kD homodimeric disulfide-linked protein consisting of two 120 amino acids. Biological Activity: The ED50 was determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells is 1.0-2.0ug/ml. Amino Acid Sequence: APLATRQGK RPSKNLKARC SRKALHVNFK APSATRQGK RPSKNLKARC SRKALHVNFK DMGWDDMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ TLMNSMDPES TPPTCCVPTR LSPISILFID SANNVVYKQY EDMVVESCGC R Crossreactivity: Mouse Quality Control: Verified by N-terminal and Mass Spectrometry analyses (when applicable). Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Do not vortex. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA). Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice