FGF-18, Recombinant, Human (Fibroblast Growth Factor-18, zFGF5, FGFI)

Size

£338.00

SKU:: 143321
Purity:: ≥95% by SDS-PAGE gel and HPLC analyses. Endotoxin: ≤0.1ng/ug (<1EU/ug).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from10mM Tris pH8.0, 20mM sodium chloride. Reconstitute in sterile dH20 to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: FGF-18 is a heparin binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-18 is an essential regulator of long bone and calvarial development. FGF-18 signals through FGFR 1c, 2c, 3c, and 4. Recombinant E.coli derived human FGF-18 is a 20.1kD protein consisting of 173 amino acid residues, resulting from c-terminal truncation of the full length protein. Amino Acid Sequence: AEENVDFRI HVENQTRARD DVSRKQLRLY QLYSRTSGKH IQVLGRRISA RGEDGDKYAQ LLVETDTFGS QVRIKGKETE FYLCMNRKGK LVGKPDGTSK ECVFIEKVLE NNYTALMSAK YSGWYVGFTK KGRPRKGPKT RENQQDVHFM KRYPKGQPEL QKPFKYTTVT KRSR Verified by UV Spectroscopy and/or SDS-PAGE gel. Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel. Biological Activity: ASSAY #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ~0.5ng/ml, corresponding to a specific activity of ~2x10e6 units/mg. ASSAY #2: The ED50 as determined by cell proliferation assay using balb/c 3T3 cells. The expected ED50 is <10.0ng/ml, corresponding to a specific activity of ≥1x10e5 units/mg. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice