Vasoactive Intestinal Peptide (VIP)

Size

£482.00

SKU:: 142789
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa29-165 with an N-terminal His-tag from human VIP, expressed in E.coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:200 Western Blot: 1:100-1:400 Immunocytochemistry: 1:100-1:500 Immunohistochemistry (frozen): 1:100-1:500 Immunohistochemistry (paraffin sections): 1:50-1:200 Optimal dilutions to be determined by the researcher. AA Sequence: MGHHHHHHSG SEF- YR APSALRLGDR IPFEGANEPD QVSLKEDIDM LQNALAENDT PYYDVSRNAR HADGVFTSDF SKLLGQLSAK KYLESLMGKR VSSNISEDPV PVKRHSDAVF TDNYTRLRKQ MAVKKYLNSI LNGKRSSEGE SPDFP Positive Control (Supplied): 142789A: Vasoactive Intestinal Peptide, Positive Control (VIP) Suppled as a recombinant protein (aa29-165) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice