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142415

T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT)

Size

£522.00

SKU:
142415
Purity:
Purified by affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to aa28-246 with two N-Terminal Tags, His-Tag and T7-Tag from mouse TIGIT, expressed in E. coli.
Format:
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:
Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and, Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:5000 Western Blot: 1:50-1:400 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-1:100 Immunocytochemistry: 1:50-1:500 Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GTI DTKRNISAEE GGSVILQCHF SSDTAEVTQV DWKQQDQLLA IYSVDLGWHV ASVFSDRVVP GPSLGLTFQS LTMNDTGEYF CTYHTYPGGI YKGRIFLKVQ ESSDDRNGLA QFQTAPLGGT MAAVLGLICL MVTGVTVLAR KDKSIRMHSI ESGLGRTEAE PQEWNLRSLS SPGSPVQTQT APAGPCGEQA EDDYADPQEY FNVLSYRSLE SFIAVS Positive Control (Supplied): 152178A: Suppled as a recombinant protein (aa28-246) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice