142221
Secreted Frizzled Related Protein 5 (SFRP5)
Size
£514.00
- SKU:
- 142221
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa45-162 of rat SFRP5 with N-terminal His-Tag, expressed in E.coli.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
- Extra Details:
- SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.Highly expressed in the retinal pigment epithelium (RPE) and pancreas. Weak expression in heart, liver and muscle. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-200 Western Blot: 1:100-400 Immunocytochemistry: 1:100-500 Immunohistochemistry (frozen): 1:100-500 Immunohistochemistry (paraffin sections): 1:50-200 Optimal dilutions to be determined by the researcher. Positive Control (supplied): 142221A Suppled as a recombinant in loading buffer. DAB: 10ul/well ECL: 5ul/well AA Sequence: GHHHHHHSGSEF-SKPPQC LDIPADLPLC HTVGYKRMRL PNLLEHESLA EVKQQASSWL PLLAKRCHSD TQVFLCSLFA PVCLDRPIYP CRSLCEAVRA GCAPLMEAYG FPWPEMLHCH KFPLDNDLCI AV Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice