S100 Calcium Binding Protein B (S100B, S100-B, NEF, S100Beta)

Size

£495.00

SKU:: 142203
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Guinea Pig
Immunogen:: E. coli-expressed recombinant rabbit S100B with N-terminal His-tag
Format:: Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-200 Western Blot: 1:100-400 Immunocytochemistry: 1:100-500 Immunohistochemistry: 1:100-500 (frozen) Immunohistochemistry: 1:50-200 (paraffin sections) Optimal dilutions to be determined by the researcher. AA Sequence: MGHHHHHHSGSEF-MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE Positive Control (Supplied): 142203A: Suppled as a recombinant protein S100B (Met1~Glu92) in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, BPB 0.01%, 0.02% sodium azide, 50% glycerol, DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.