Phospholipase A2, Group IID (PLA2G2D, PLA2G2D, sPLA2S, s-PLA2, sPLA2, Secreted Phospholipase A2)

Size

£495.00

SKU:: 141871
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Leu21-Cys144 with an N-terminal His-Tag from mouse PLA2G2D, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.05-2ug/ml Western Blot: 0.5-2ug/ml Immunohistochemistry (frozen): 5-20ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Positive Control Included: 141871A: Phospholipase A2, Group IID, Positive Control (PLA2G2D, PLA2G2D, sPLA2S, s-PLA2, sPLA2, Secreted Phospholipase A2) Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 0.02% BPB, 50mM DTT, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.