Milk Fat Globule EGF Factor 8 (MFGE8, MFG-E8, BA46, EDIL1, OAcGD3S, SPAG10, hP47, Lactadherin, Sperm Associated Antigen 10, Sperm Surface Protein hP47)

Size

£466.00

SKU:: 141446
Purity:: Purified by affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: E. coli-expressed recombinant protein corresponding to aa159-417 of mouse MFGE8 with an N-terminal His tag.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:1:200 Western Blot: 1:50-1:400 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-1:100 Immunocytochemistry: 1:50-1:500 Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MGHHHHHHSGSEF- IA DSQISASSVY MGFMGLQRWG PELARLYRTG IVNAWTASNY DSKPWIQVNL LRKMRVSGVM TQGASRAGRA EYLKTFKVAY SLDGRKFEFI QDESGGDKEF LGNLDNNSLK VNMFNPTLEA QYIKLYPVSC HRGCTLRFEL LGCELHGCSE PLGLKNNTIP DSQMSASSSY KTWNLRAFGW YPHLGRLDNQ GKINAWTAQS NSAKEWLQVD LGTQRQVTGI ITQGARDFGH IQYVASYKVA HSDDGVQWTV YEEQGSS Positive Control Included: 141446A: Milk Fat Globule EGF Factor 8, Positive Control Suppled as a recombinant protein (aa159-417) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice