141049
Interleukin 35 (IL35)
Size
£499.00
- SKU:
- 141049
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- IL35 complex composed of IL27B subunit (Arg21-Lys229) and the mature form of human IL12A (Arg23-Ser219) linked by a polypeptide linker (GGGGS)3 expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:1:200 Western Blot: 1:50-1:1:400 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-1:100 Immunocytochemistry: 1:50-1:500 Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MGHHHHHHSG SEFELRRQ-RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGKS GGGGSGGGGS GGGGSRNLPV ATPDPGMFPC LHHSQNLLRA VSNMLQKARQ TLEFYPCTSE EIDHEDITKD KTSTVEACLP LELTKNESCL NSRETSFITN GSCLASRKTS FMMALCLSSI YEDLKMYQVE FKTMNAKLLM DPKRQIFLDQ NMLAVIDELM QALNFNSETV PQKSSLEEPD FYKTKIKLCI LLHAFRIRAV TIDRVTSYLN AS Positive Control Included: 141049A: IL;35, Positive Control Suppled as a recombinant IL35 protein in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice