140945
Interleukin 1 Beta (IL1b, IL1-B, IL1-Beta, IL1F2, IL-1b, Interleukin-1 Family Member 2)
Size
£493.00
- SKU:
- 140945
- Purity:
- Purified by affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Guinea Pig
- Immunogen:
- Recombinant protein coresponding to aa118-268 from rabbit IL1b expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
- Extra Details:
- Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:200 Western Blot: 1:100-1:400 Immunohistochemistry (frozen): 1:100-1:500 Immunohistochemistry (Paraffin): 1:50-1:200 Immunocytochemistry: 1:100-1:500 Optimal dilutions to be determined by the researcher. Positive Control (supplied): 140945A: Suppled as a recombinant protein (aa118-268) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Amino Acid Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag expressed in E. coli. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VRS LHCRLQDAQQ KSLVLSGTYE LKALHLNAEN LNQQVVFSMS FVQGEESNDK IPVALGLRGK NLYLSCVMKD DKPTLQLESV DPNRYPKKKM EKRFVFNKIE IKDKLEFESA QFPNWYISTS QTEYMPVFLG NNSGGQDLID FSMEFVSS Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice