IDS (Iduronate-2-Sulfatase, Alpha-L-iduronate Sulfate Sulfatase, Idursulfase, SIDS)

Size

£537.00

SKU:: 140754
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa180-448 with an N-Terminal His-Tag from mouse IDS, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:200 Western Blot: 1:50-1:400 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-1:100 Immunocytochemistry: 1:50-1:500 Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MGHHHHHHSG SEF-L HANLLCPVDV ADVPEGTLPD KQSTEEAIRL LEKMKTSASP FFLAVGYHKP HIPFRYPKEF QKLYPLENIT LAPDPHVPDS LPPVAYNPWM DIREREDVQA LNISVPYGPI PEDFQRKIRQ SYFASVSYLD TQVGHVLSAL DDLRLAHNTI IAFTSDHGWA LGEHGEWAKY SNFDVATRVP LMLYVPGRTA PLPAAGQKLF PYRDPFDPAS DWMDAGRHTE DLVELVSLFP TLAGLAGLPV PPRCPIPSFH VELCREGQNL QKHLQLHD Positive Control (Supplied): 140754A: Suppled as a recombinant protein (aa180-448) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice