140628
HMOX1, Decycling (Heme Oxygenase-1, Heme Oxygenase 1, HO-1, HO, HO1)
Size
£482.00
- SKU:
- 140628
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant HMOX1, Decycling (Leu61~Ile172) with two N-terminal tags, His-tag and S-Tag, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:200 Western Blot: 1:50-1:400 Immunocytochemistry: 1:50-1:500 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (paraffin sections): 1:10-1:100 Optimal dilutions to be determined by the researcher. Amino Acid Sequence: HO1 (Leu61~Ile172); MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- LEEEIERNKE SPVFAPVYFP EELHRKAALE QDLAFWYGPR WQEVIPYTPA MQRYVKRLHE VGRTEPELLV AHAYTRYLGD LSGGQVLKKI AQKALDLPSS GEGLAFFTFP NI Positive Control (Supplied): 140628A: Suppled as a recombinant protein (aa61-172) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice