GDNF (Glial Cell Line-derived Neurotrophic Factor, hGDNF, Astrocyte-derived Trophic Factor, ATF)

Size

£581.00

SKU:: 140424
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein GDNF (Asp79-Leu217) with a N-terminal His-Tag from mouse GDNF, expressed in E.coli.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:200 Western Blot: 1:100-1:400. A band is detected at ~19kD. Immunocytochemistry: 1:100-1:500 Immunohistochemistry (frozen): 1:100-1:500 Immunohistochemistry (Paraffin): 1:50-1:200 Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MGHHHHHHSGS-DS NMPEDYPDQF DDVMDFIQAT IKRLKRSPDK QAAALPRRER NRQAAAASPE NSRGKGRRGQ RGKNRGCVLT AIHLNVTDLG LGYETKEELI FRYCSGSCES AETMYDKILK NLSRSRRLTS DKVGQACCRP VAFDDDL Positive Control Included: 140424A: GDNF Positive Control Suppled as a recombinant protein (aa79-217) in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol. DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice