140116
ESAM (Endothelial Cell-selective Adhesion Molecule, UNQ220/PRO246, W117m)
Size
£499.00
- SKU:
- 140116
- Purity:
- Purified by affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa31-390 of human ESAM expressed in E. coli with an N-terminal His-Tag.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
- Extra Details:
- Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:200 Western Blot: 1:100-1:400 Immunohistochemistry (frozen): 1:100-1:500 Immunohistochemistry (Paraffin): 1:50-1:200 Immunocytochemistry: 1:100-1:500 Optimal dilutions to be determined by the researcher. AA Sequence: MGHHHHHHSGSEF-LQLHLPANRL QAVEGGEVVL PAWYTLHGEV SSSQPWEVPF VMWFFKQKEK EDQVLSYING VTTSKPGVSL VYSMPSRNLS LRLEGLQEKD SGPYSCSVNV QDKQGKSRGH SIKTLELNVL VPPAPPSCRL QGVPHVGANV TLSCQSPRSK PAVQYQWDRQ LPSFQTFFAP ALDVIRGSLS LTNLSSSMAG VYVCKAHNEV GTAQCNVTLE VSTGPGAAVV AGAVVGTLVG LGLLAGLVLL YHRRGKALEE PANDIKEDAI APRTLPWPKS SDTISKNGTL SSVTSARALR PPHGPPRPGA LTPTPSLSSQ ALPSPRLPTT DGAHPQPISP IPGGVSSSGL SRMGAVPVMV PAQSQAGSLV Positive Control Included: 140116A: ESAM, Positive Control Suppled as a recombinant protein (aa31-390) in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol. DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice