BNP (Natriuretic Peptides B, Gamma-brain Natriuretic Peptide, NPPB)

Size

£462.00

SKU:: 139466
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa27-134 from human BNP with a N-terminal His-tag, expressed in E. coli
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
Extra Details:: Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:200 Western Blot: 1:100-1:400 Immunohistochemistry (Paraffin): 1:50-1:200 Immunohistochemistry: 1:100-1:500 (frozen) Immunocytochemistry: 1:100-1:500 Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MGHHHHHHSGSEF-HPLG SPGSASDLET SGLQEQRNHL QGKLSELQVE QTSLEPLQES PRPTGVWKSR EVATEGIRGH RKMVLYTLRA PRSPKMVQGS GCFGRKMDRI SSSSGLGCKV LRRH Positive Control Included: 139466A: BNP, Positive Control Suppled as a recombinant protein (aa27-137) in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol. DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.