139217
Angiopoietin 1 (ANG-1, ANGPT1, KIAA0003, Angiopoietin-1)
Size
£438.00
- SKU:
- 139217
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa21-228 human ANGPT1, fused with N-terminal His-Tag, expressed in E.coli.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
- Extra Details:
- Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1.Angiopoietin-1 (Ang1) and Angiopoietin-2 (Ang2) are ligands that regulate tyrosine phosphorylation of the Tie2/Tek receptor on endothelial cells. Proper regulation of Tie2/Tek is required for normal vascular development, by regulating vascular remodeling and endothelial cell interactions. Antiopoietin-2 (Ang2) is an antagonist of Ang1 that competes for binding of the Tie2 receptor and blocks Ang-1 induced Tie2 phosphorylation during vasculogenesis. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:100-200 Western Blot: 1:100-400 Immunocytochemistry: 1:100-500 Immunohistochemistry (frozen): 1:100-500 Immunohistochemistry (paraffin sections): 1:50-200 Optimal dilutions to be determined by the researcher. Positive Control (supplied): 139217A Suppled as a recombinant in loading buffer. DAB: 10ul/well ECL: 5ul/well AA Sequence: MGHHHHHHSG S- NQRRSPENSG RRYNRIQHGQ CAYTFILPEH DGNCRESTTD QYNTNALQRD APHVEPDFSS QKLQHLEHVM ENYTQWLQKL ENYIVENMKS EMAQIQQNAV QNHTATMLEI GTSLLSQTAE QTRKLTDVET QVLNQTSRLE IQLLENSLST YKLEKQLLQQ TNEILKIHEK NSLLEHKILE MEGKHKEELD TLKEEKENLQ GLVTRQTY Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice