RNF181, Recombinant, Human, aa1-153, His-Tag (E3 Ubiquitin-protein Ligase RNF181, HSPC238)

Size

£512.00

SKU:: 138729
Molecular Weight:: 20.3
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.
Extra Details:: E3 ubiquitin-protein ligase RNF181, also known as RNF181, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Source: Recombinant protein corresponding to aa1-153 from human RNF181 at N-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~20.3kD (176aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGS RGSQAELKCP VCLLEFEEEE TAIEMPCHHL FHSSCILPWL SKTNSCPLCR YELPTDDDTY EEHRRDKARK QQQQHRLENL HGAMYT MASYFDE HDCEPSDPEQ ETRTNMLLEL ARSLFNRMDF EDLGLVVDWD HHLPPPAAKT VVENLPRTVI Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.