RNASE3, Recombinant, Human, aa28-160, His-Tag (Eosinophil Cationic Protein, ECP, RNS3, Ribonuclease 3)

Size

£512.00

SKU:: 138728
Molecular Weight:: 19.9
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.4M urea, 10% glycerol.
Extra Details:: RNASE3 is cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. It possesses a wide variety of biological activities. This protein exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains and promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content Recombinant protein corresponding to aa28-160 from human RNASE3 at N-terminal, fused to His-tag expressed in E. coli. Accession: AAH96060 Molecular Weight: ~19.9kD (171aa) Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRP PQFTRAQWFA IQHISLNPPR CTIAMRAINN YRWRCKNQNT FLRTTFANVV NVCGNQSIRC PHNRTLNNCH RSRFRVPLLH CDLINPGAQN ISNCRYADRP GRRFYVVACD NRDPRDSPRY PVVPVHLDTT I Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.