POLR3H, Recombinant, Human, aa1-204, His-Tag (DNA-directed RNA Polymerase III Subunit RPC8, RPC22.9, RPC8)

Size

£512.00

SKU:: 138717
Molecular Weight:: 25.3
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.1M sodium chloride, 1mM DTT, 10% glycerol.
Extra Details:: POLR3H, also as known as A1665 and RPC8, belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. POLR3H is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. POLR3H acts as nuclear and cytosolic DNA sensor involved in innate immune response. Source: Recombinant protein corresponding to aa1-204 from human POLR3H at N-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~25.3kD (227aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGS TKVHFRCVVF HPFLDEILIG KIKGCSPEGV HVSLGFFDDI LIPPESLQQP AKFDEAEQVW VWEYETEEGA HDLYMDTGEE IRFRVVDESF MFVLVEM VDTVRIPPWQ FERKLNDSIA EELNKKLANK VVYNVGLCIC LFDITKLEDA YVFPGDGASH VDTSPTGPSS ADATTSSEEL PKKEAPYTLV GSISEPGLGL LSWWTSN Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.